Difference between revisions of "Part:BBa K2148008"

 
 
Line 5: Line 5:
 
This is a coding DNA sequence (CDS) for a yellow fluorescent protein which is codon-optimised for Chlamydomonas reinhardtii chloroplast. It is coded as a CDS/CTAG Phytobrick.
 
This is a coding DNA sequence (CDS) for a yellow fluorescent protein which is codon-optimised for Chlamydomonas reinhardtii chloroplast. It is coded as a CDS/CTAG Phytobrick.
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
This is a level-0 Phytobrick conforming to the Phytobrick standard. It is coded as a CDS/CTAG Phytobrick and can be used with other level-0 Phytobricks to create a transcriptional unit that expresses a yellow Venus fluorescent protein for Chlamydomonas reinhardtii.
  
 
<!-- -->
 
<!-- -->
Line 17: Line 17:
 
<partinfo>BBa_K2148008 parameters</partinfo>
 
<partinfo>BBa_K2148008 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
===Characterisation===
 +
 +
===References===
 +
Rasala, B. A., Barrera, D. J., Ng, J., Plucinak, T. M., Rosenberg, J. N., Weeks, D. P., Oyler, G. A., Peterson, T. C., Haerizadeh, F. and Mayfield, S. P. (2013), Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii. Plant J, 74: 545–556. doi:10.1111/tpj.12165

Latest revision as of 09:43, 11 October 2016


mVenus C.reinhardtii-CO Yellow FP

This is a coding DNA sequence (CDS) for a yellow fluorescent protein which is codon-optimised for Chlamydomonas reinhardtii chloroplast. It is coded as a CDS/CTAG Phytobrick.

Usage and Biology

This is a level-0 Phytobrick conforming to the Phytobrick standard. It is coded as a CDS/CTAG Phytobrick and can be used with other level-0 Phytobricks to create a transcriptional unit that expresses a yellow Venus fluorescent protein for Chlamydomonas reinhardtii.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 668
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 668
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 668
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 668
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 668
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterisation

References

Rasala, B. A., Barrera, D. J., Ng, J., Plucinak, T. M., Rosenberg, J. N., Weeks, D. P., Oyler, G. A., Peterson, T. C., Haerizadeh, F. and Mayfield, S. P. (2013), Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii. Plant J, 74: 545–556. doi:10.1111/tpj.12165