Difference between revisions of "Part:BBa K2148009"

Latest revision as of 22:43, 14 October 2016

C.reinhardtii CO green fluorescent protein

A coding DNA sequence for green fluorescent protein (GFP) flanked by fusion site for a CDS/CTAG Phytobrick, which can be used as a reporter gene.

Usage and Biology

Green fluorescent protein can be used as a reporter. Coded as a CDS/CTAG Phytobrick conforming to the Phytobrick standard, this part can be used with other level-0 Phytobricks to form the desired transcriptional unit.

It is an optimised form of the original GFP gene, BBa_E0040, which we codon-optimised for the chloroplasts of Chlamydomonas reinhardtii.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Characterisation

References

Davis, S.J. & Vierstra, R.D. Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants. Plant Mol Biol (1998) 36: 521. doi:10.1023/A:1005991617182

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 Green fluorescent protein can be used as a reporter. Coded as a CDS/CTAG Phytobrick conforming to the Phytobrick standard, this part can be used with other level-0 Phytobricks to form the desired transcriptional unit.
+It is an optimised form of the original GFP gene, <html><a target="_blank" href="https://parts.igem.org/Part:BBa_E0040">BBa_E0040</a></html>, which we codon-optimised for the chloroplasts of <i>Chlamydomonas reinhardtii</i>.
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 ===References===
-Davis, S.J. & Vierstra, R.D.,Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants, Plant Mol Biol (1998) 36: 521. doi:10.1023/A:1005991617182
+Davis, S.J. & Vierstra, R.D. Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants. Plant Mol Biol (1998) 36: 521. doi:10.1023/A:1005991617182