Difference between revisions of "Part:BBa K2148009"
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A coding DNA sequence for green fluorescent protein (GFP) flanked by fusion site for a CDS/CTAG Phytobrick, which can be used as a reporter gene. | A coding DNA sequence for green fluorescent protein (GFP) flanked by fusion site for a CDS/CTAG Phytobrick, which can be used as a reporter gene. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | Green fluorescent protein can be used as a reporter. Coded as a CDS/CTAG Phytobrick conforming to the Phytobrick standard, this part can be used with other level-0 Phytobricks to form the desired transcriptional unit. | ||
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+ | It is an optimised form of the original GFP gene, <html><a target="_blank" href="https://parts.igem.org/Part:BBa_E0040">BBa_E0040</a></html>, which we codon-optimised for the chloroplasts of <i>Chlamydomonas reinhardtii</i>. | ||
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<partinfo>BBa_K2148009 parameters</partinfo> | <partinfo>BBa_K2148009 parameters</partinfo> | ||
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+ | ===Characterisation=== | ||
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+ | ===References=== | ||
+ | Davis, S.J. & Vierstra, R.D. Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants. Plant Mol Biol (1998) 36: 521. doi:10.1023/A:1005991617182 |
Latest revision as of 22:43, 14 October 2016
C.reinhardtii CO green fluorescent protein
A coding DNA sequence for green fluorescent protein (GFP) flanked by fusion site for a CDS/CTAG Phytobrick, which can be used as a reporter gene.
Usage and Biology
Green fluorescent protein can be used as a reporter. Coded as a CDS/CTAG Phytobrick conforming to the Phytobrick standard, this part can be used with other level-0 Phytobricks to form the desired transcriptional unit.
It is an optimised form of the original GFP gene, BBa_E0040, which we codon-optimised for the chloroplasts of Chlamydomonas reinhardtii.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterisation
References
Davis, S.J. & Vierstra, R.D. Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants. Plant Mol Biol (1998) 36: 521. doi:10.1023/A:1005991617182