Difference between revisions of "Part:BBa K2114019:Design"
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===Source=== | ===Source=== | ||
| − | The cgeA gene comes from the genome of B. subtilis. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare). | + | The cgeA gene comes from the genome of <i>B. subtilis</i>. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare). |
===References=== | ===References=== | ||
Latest revision as of 06:37, 20 October 2016
GST_HA_G4S_CgeA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 688
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1008
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 85
Design Notes
Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR.
Source
The cgeA gene comes from the genome of B. subtilis. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare).