Difference between revisions of "Part:BBa K2132005:Design"
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===Source=== | ===Source=== | ||
| − | The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824. | + | The sequence of '' hydA '' hydrogenase gene is from the chromosome of '' Clostridium acetobutylicum '' ATCC 824. |
===References=== | ===References=== | ||
Paul W. King, Matthew C. Posewitz, Maria L. Ghirardi, Michael Seibert. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system.[J]. Journal of Bacteriology, 2006, 188(6):2163-72. | Paul W. King, Matthew C. Posewitz, Maria L. Ghirardi, Michael Seibert. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system.[J]. Journal of Bacteriology, 2006, 188(6):2163-72. | ||
Latest revision as of 13:02, 9 October 2016
HydA with SpyTag, Histag and TEV site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 790
Illegal AgeI site found at 1300 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1005
Design Notes
We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag.
Source
The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824.
References
Paul W. King, Matthew C. Posewitz, Maria L. Ghirardi, Michael Seibert. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system.[J]. Journal of Bacteriology, 2006, 188(6):2163-72.