Difference between revisions of "Part:BBa K2070016"

 
 
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__NOTOC__
 
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<partinfo>BBa_K2070005 short</partinfo>
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<partinfo>BBa_K2070016 short</partinfo>
  
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To be edited
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This part contains an ECF16 coding region downstream of a medium rbs(BBa_B0032) and strong promoter (BBa_J23101).
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The ecf16 generated can activate the ecf16 promoter (<partinfo>BBa_K2070000</partinfo>).
  
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===Usage and Biology===
 
===Usage and Biology===
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[[File:T--UT-Tokyo--BBa K2070000--sigma1601.png|thumb|centre|500px|'''Photograph of plates containing cells transformed with sigma16 related constructs under blue light.'''
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From bottom left: BBa_K2070006 (ECF sigma16 inducible GFP reporter), BBa_K2070026 (Constitutive ecf16 generator with ecf 16 inducible GFP reporter), BBa_K2070016 (Constitutive ECF sigma16 generator). As only cells with BBa_K2070026 is shown to fluorescence, it can be suggested that the devices works as expected.]]
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2070016 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2070005 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2070005 parameters</partinfo>
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<partinfo>BBa_K2070016 parameters</partinfo>
 
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Latest revision as of 07:04, 25 October 2016


Constitutive ecf16 generator

This part contains an ECF16 coding region downstream of a medium rbs(BBa_B0032) and strong promoter (BBa_J23101). The ecf16 generated can activate the ecf16 promoter (BBa_K2070000).

Usage and Biology

Photograph of plates containing cells transformed with sigma16 related constructs under blue light. From bottom left: BBa_K2070006 (ECF sigma16 inducible GFP reporter), BBa_K2070026 (Constitutive ecf16 generator with ecf 16 inducible GFP reporter), BBa_K2070016 (Constitutive ECF sigma16 generator). As only cells with BBa_K2070026 is shown to fluorescence, it can be suggested that the devices works as expected.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 434
  • 1000
    COMPATIBLE WITH RFC[1000]