Difference between revisions of "Part:BBa K2070001:Design"

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===Design Notes===
 
===Design Notes===
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For detail, see our wiik
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The part sequence was designed with reference to the amnio acid sequence of ECF sigma provided by Rhodius V.A et al (2014). Specific sequences were selected so that no illegal cut would be introduced and so that parts could be synthesised by IDT gene fragments.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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Rhodius, V. A., Segall-Shapiro, T. H., Sharon, B. D., Ghodasara, A., Orlova, E., Tabakh, H., Voigt, C. A. (2014, 04). Design of orthogonal genetic switches based on a crosstalk map of  s, anti- s, and promoters. Molecular Systems Biology, 9(1), 702-702. doi:10.1038/msb.2013.58

Latest revision as of 16:01, 30 October 2016


ecf16


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 177
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 372
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part sequence was designed with reference to the amnio acid sequence of ECF sigma provided by Rhodius V.A et al (2014). Specific sequences were selected so that no illegal cut would be introduced and so that parts could be synthesised by IDT gene fragments.

Source

Pseudomonas entomophila L48

References

Rhodius, V. A., Segall-Shapiro, T. H., Sharon, B. D., Ghodasara, A., Orlova, E., Tabakh, H., Voigt, C. A. (2014, 04). Design of orthogonal genetic switches based on a crosstalk map of s, anti- s, and promoters. Molecular Systems Biology, 9(1), 702-702. doi:10.1038/msb.2013.58