Difference between revisions of "Part:BBa K2114007:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR. | |
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===Source=== | ===Source=== | ||
Latest revision as of 02:02, 9 October 2016
aGFPnano_HA_G4S_CotG
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR.
Source
The cotG gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived from camelid heavy-chain antibodies.