Difference between revisions of "Part:BBa K2066037"
 
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<partinfo>BBa_K2066037 short</partinfo>
 
<partinfo>BBa_K2066037 short</partinfo>
  
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<p>This part is part of William and Mary iGEM 2016's library of IPTG-inducible RBS characterization parts. It contains the RBS BBa_B0031. </p>
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This is the pACT-Tet sequence from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper on the UNS standard. This part consists of the endogenous nitrogen regulatory promoter, the NRII mutant  BBa_K2066112 and tetR. This is meant for use in Tet based synthetic enhancer systems, such as the [http://2016.igem.org/Team:William_and_Mary/Synthetic_Enhancer William and Mary 2016]
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<p>The part codes for the expression of a superfolder GFP and is regulated by a lacI-repressible plLacO-1 Promoter. By adding IPTG one should be able to induce the expression of sfGFP and compare the induction curve to other IPTG-inducible RBS characterization parts from our library to determine the relative strengths of different RBS sequences across an induction curve.</p>
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<p>We have included the self-cleaving ribozyme RiboJ immediately upstream of the RBS sequence in order to buffer against translational influence from the 5' untranslated region conferred to the transcript by the promoter sequence.</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 04:50, 29 October 2016


pACT-Tet on UNS Standard

This is the pACT-Tet sequence from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper on the UNS standard. This part consists of the endogenous nitrogen regulatory promoter, the NRII mutant BBa_K2066112 and tetR. This is meant for use in Tet based synthetic enhancer systems, such as the [http://2016.igem.org/Team:William_and_Mary/Synthetic_Enhancer William and Mary 2016]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 969
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]