Difference between revisions of "Part:BBa K2027012"

Latest revision as of 15:15, 27 October 2016

Mutant Feedback-Resistant Version of MelA Tyrosinase

Mutant Feedback-Resistant Version of MelA Tyrosinase. The MutmelA gene endogenous to Rhizobium etli encodes a feedback resistant tyrosinase that has been shown to be effective when transfected into E. coli. [8] Based on the work of MI Chávez-Béjar, our team sought to increase the production of L-tyrosine in E. coli through metabolic engineering to shuttle the flow of carbon from the cells’ central metabolism into the shikimate pathway.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1379
Illegal AgeI site found at 1661
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1231
Illegal BsaI.rc site found at 25
Illegal BsaI.rc site found at 536

Learn more here: http://2016.igem.org/Team:Stanford-Brown/SB16_BioMembrane_UV

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2027012 short</partinfo>
-Mutant Feedback-Resistant Version of MelA Tyrosinase
+Mutant Feedback-Resistant Version of MelA Tyrosinase. The MutmelA gene endogenous to Rhizobium etli encodes a feedback resistant tyrosinase that has been shown to be effective when transfected into E. coli. [8] Based on the work of MI Chávez-Béjar, our team sought to increase the production of L-tyrosine in E. coli through metabolic engineering to shuttle the flow of carbon from the cells’ central metabolism into the shikimate pathway.
 <!-- Add more about the biology of this part here
@@ Line 11: / Line 11: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K2027012 SequenceAndFeatures</partinfo>
+Learn more here: http://2016.igem.org/Team:Stanford-Brown/SB16_BioMembrane_UV
 <!-- Uncomment this to enable Functional Parameter display