Difference between revisions of "Part:BBa K2151005"
 
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<p>This part consists of E5501 (GFP with medium ribosome binding site) under the control of p32, a strong constitutive bacterial promoter. Its intended use is to serve as a promoter-reporter construct in lactic acid bacteria. p32 is used as a promoter in expression vectors functioning within <i>Streptococcus thermophilus</i> </p>
 
<p>This part consists of E5501 (GFP with medium ribosome binding site) under the control of p32, a strong constitutive bacterial promoter. Its intended use is to serve as a promoter-reporter construct in lactic acid bacteria. p32 is used as a promoter in expression vectors functioning within <i>Streptococcus thermophilus</i> </p>
  
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  <p>We used p32 as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of p32 using GFP revealed promoter strength on par with strong Anderson promoters (81% as strong as J23100, 115% as strong as J23101).</p></p>
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  <p>We used p32 as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of p32 using GFP revealed promoter strength on par with strong Anderson promoters (81% as strong as J23100, 115% as strong as J23101).</p>
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[[File:T--Glasgow--B0032 Measurement.jpg]]
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<b>Figure 2:</b> Mean fluorescence values for E5501 under the control of various promoters. Red represents S. thermophilus promoters, blue represents Anderson family promoters. Error bars represent standard deviation for each set of samples. Promoters are ordered according to their strength when expressing I13500.
  
 
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Latest revision as of 05:05, 29 October 2016


p32-E5501: GFP under control of strong constitutive promoter

This part consists of E5501 (GFP with medium ribosome binding site) under the control of p32, a strong constitutive bacterial promoter. Its intended use is to serve as a promoter-reporter construct in lactic acid bacteria. p32 is used as a promoter in expression vectors functioning within Streptococcus thermophilus

We used p32 as a candidate promoter to drive gene expression in both Streptococcus thermophilus and E. coli. Quantification of p32 using GFP revealed promoter strength on par with strong Anderson promoters (81% as strong as J23100, 115% as strong as J23101).

T--Glasgow--B0032 Measurement.jpg

Figure 2: Mean fluorescence values for E5501 under the control of various promoters. Red represents S. thermophilus promoters, blue represents Anderson family promoters. Error bars represent standard deviation for each set of samples. Promoters are ordered according to their strength when expressing I13500.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 721