Difference between revisions of "Part:BBa K2151002"

Latest revision as of 04:59, 29 October 2016

p32, strong constitutive S. thermophilus promoter

This part consists of p32, a strong constitutive bacterial promoter, contained within pSB1C3. Its intended use is to promote transcription of an inserted gene. p32 is used as a promoter in expression vectors functioning within Streptococcus thermophilus

We used p32 as a candidate promoter to drive gene expression in both Streptococcus thermophilus and E. coli. Quantification of p32 using GFP revealed promoter strength on par with strong Anderson promoters (81% as strong as J23100, 115% as strong as J23101).

Figure 1: Mean fluorescence values for I13500 under the control of various promoters. Orange represents S. thermophilus promoters, blue represents Anderson family promoters. Error bars represent standard deviation for each set of samples.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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   <p>We used p32 as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of p32 using GFP revealed promoter strength on par with strong Anderson promoters (81% as strong as J23100, 115% as strong as J23101).</p>
+[[File:T--Glasgow--B0034 Measurement.jpg]]
+<p><b>Figure 1: </b>Mean fluorescence values for I13500 under the control of various promoters. Orange represents <i>S. thermophilus</i> promoters, blue represents Anderson family promoters. Error bars represent standard deviation for each set of samples.</p>
 <!-- Add more about the biology of this part here