Difference between revisions of "Part:BBa K2151000:Design"

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<ul>
 
<ul>
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<li>Sequence obtained from 'ISOLATION AND CHARACTERIZATION OF CHROMOSOMAL PROMOTERS OF STREPTOCOCCUS-SALIVARIUS SUBSP THERMOPHILUS'.</li>
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<li>Sequence obtained from 'Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene'.</li>
  
 
<li>Synthesized by IDT as an oligo-nucleotide</li>
 
<li>Synthesized by IDT as an oligo-nucleotide</li>
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===References===
 
===References===
  
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  <p>Slos, P., Bourquin, J. C., Lemoine, Y. and Mercenier, A. (1991) 'ISOLATION AND CHARACTERIZATION OF CHROMOSOMAL PROMOTERS OF STREPTOCOCCUS-SALIVARIUS SUBSP THERMOPHILUS', Applied and Environmental Microbiology, 57(5), pp. 1333-1339.</p>
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  <p>Chouayekh, H., Serror, P., Boudebbouze, S. and Maguin, E. (2009) 'Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene', Fems Microbiology Letters, 293(2), pp. 232-239.</p>

Latest revision as of 20:55, 8 October 2016


pHLBA, strong constitutive S. thermophilus promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was not altered for use in E. coli as the aim was to achieve expression in S. thermophilus.

Source

  • Sequence obtained from 'Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene'.
  • Synthesized by IDT as an oligo-nucleotide

References

Chouayekh, H., Serror, P., Boudebbouze, S. and Maguin, E. (2009) 'Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene', Fems Microbiology Letters, 293(2), pp. 232-239.