Difference between revisions of "Part:BBa K2151001"
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<p>This part consists of p25, a medium constitutive bacterial promoter, contained within pSB1C3. Its intended use is to promote transcription of an inserted gene. p25 was initially identified in a promoter probe survey of the <i>Streptococcus thermophilus</i> genome, alongside weaker promoters p8 and p20.</p> | <p>This part consists of p25, a medium constitutive bacterial promoter, contained within pSB1C3. Its intended use is to promote transcription of an inserted gene. p25 was initially identified in a promoter probe survey of the <i>Streptococcus thermophilus</i> genome, alongside weaker promoters p8 and p20.</p> | ||
| − | <p>We used p25 as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of p25 using GFP revealed promoter strength on par with medium Anderson promoters (30.8% as strong as J23100, 43.7% as strong as J23101 130% as strong as J23106).</p> | + | <p>We used p25 as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of p25 using GFP revealed promoter strength on par with medium Anderson promoters (30.8% as strong as J23100, 43.7% as strong as J23101, 130% as strong as J23106).</p> |
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| + | [[File:T--Glasgow--B0034 Measurement.jpg]] | ||
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| + | <p><b>Figure 1: </b>Mean fluorescence values for I13500 under the control of various promoters. Orange represents <i>S. thermophilus</i> promoters, blue represents Anderson family promoters. Error bars represent standard deviation for each set of samples.</p> | ||
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Latest revision as of 04:58, 29 October 2016
p25, moderate constitutive S. thermophilus promoter
This part consists of p25, a medium constitutive bacterial promoter, contained within pSB1C3. Its intended use is to promote transcription of an inserted gene. p25 was initially identified in a promoter probe survey of the Streptococcus thermophilus genome, alongside weaker promoters p8 and p20.
We used p25 as a candidate promoter to drive gene expression in both Streptococcus thermophilus and E. coli. Quantification of p25 using GFP revealed promoter strength on par with medium Anderson promoters (30.8% as strong as J23100, 43.7% as strong as J23101, 130% as strong as J23106).
Figure 1: Mean fluorescence values for I13500 under the control of various promoters. Orange represents S. thermophilus promoters, blue represents Anderson family promoters. Error bars represent standard deviation for each set of samples.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]