Difference between revisions of "Part:BBa K2151000"

Latest revision as of 04:58, 29 October 2016

pHLBA, strong constitutive S. thermophilus promoter

This part consists of pHLBA, a strong constitutive bacterial promoter, contained within pSB1C3. Its intended use is to promote transcription of an inserted gene. pHLBA was initially identified in studies of Lactobacillus bulgaricus where it drives the expression of histone-like proteins. pHLBA is likely functional in all lactic acid bacteria.

We used pHLBA as a candidate promoter to drive gene expression in both Streptococcus thermophilus and E. coli. Quantification of pHLBA using GFP revealed promoter strength on par with strong Anderson promoters (93% as strong as J23100, 132% as strong as J23101).

Figure 1: Mean fluorescence values for I13500 under the control of various promoters. Orange represents S. thermophilus promoters, blue represents Anderson family promoters. Error bars represent standard deviation for each set of samples.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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   <p>We used pHLBA as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of pHLBA using GFP revealed promoter strength on par with strong Anderson promoters (93% as strong as J23100, 132% as strong as J23101).</p>
+[[File:T--Glasgow--B0034 Measurement.jpg]]
+<p><b>Figure 1: </b>Mean fluorescence values for I13500 under the control of various promoters. Orange represents <i>S. thermophilus</i> promoters, blue represents Anderson family promoters. Error bars represent standard deviation for each set of samples.</p>
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