Difference between revisions of "Part:BBa I769009:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The TGA stop codon was mutated to TAATAA to conform with BioBrick protein coding region standards. | |
| − | + | ||
| + | There are two BioBrick restriction sites in this coding region that we need to mutate. | ||
===Source=== | ===Source=== | ||
Latest revision as of 20:43, 11 June 2007
feruloyl-CoA hydratase for vanillin biosynthesis (ech)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The TGA stop codon was mutated to TAATAA to conform with BioBrick protein coding region standards.
There are two BioBrick restriction sites in this coding region that we need to mutate.
Source
The natural source for this genetic material is Pseudomonas fluorescens; see Vanillin production using metabolically engineered Escherichia coli under non-growing conditions, Barghini et al. 2007
Genbank accession number AJ536325 (GI 40788116)