Difference between revisions of "Part:BBa K2042015"
 
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<partinfo>BBa_K2042015 short</partinfo>
 
<partinfo>BBa_K2042015 short</partinfo>
  
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Pputida promotor+igemrbs+ldh+terminqtor
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This is the sequence of a D-lactate deshydrogenase that can efficiently use NADPH and NADH as cofactors. The enzyme is under control of a Pseudomonas putida constitutive promoter and RBS. This composite part was designed to produce lactate in order to increase lactate fraction used as substrate in the Ploylactic acid (PLA) production. The part ends with the BBa_B0015 terminator.
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<partinfo>BBa_K2042015 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2042015 SequenceAndFeatures</partinfo>
  
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Inouye S, Asai Y, Nakazawa A, Nakazawa T. <br>
 
Inouye S, Asai Y, Nakazawa A, Nakazawa T. <br>
 
https://www.ncbi.nlm.nih.gov/pubmed/3011741
 
https://www.ncbi.nlm.nih.gov/pubmed/3011741
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Engineering a d-lactate dehydrogenase that can super-efficiently utilize NADPH and NADH as cofactors.<br>
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Meng H1,2, Liu P3, Sun H2,3, Cai Z2, Zhou J2, Lin J3,4, Li Y2.<br>
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https://www.ncbi.nlm.nih.gov/pubmed/27109778

Latest revision as of 19:58, 12 October 2016


LDH* Generator

This is the sequence of a D-lactate deshydrogenase that can efficiently use NADPH and NADH as cofactors. The enzyme is under control of a Pseudomonas putida constitutive promoter and RBS. This composite part was designed to produce lactate in order to increase lactate fraction used as substrate in the Ploylactic acid (PLA) production. The part ends with the BBa_B0015 terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 45
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 699
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Nucleotide sequence of a DNA segment promoting transcription in Pseudomonas putida.
Inouye S, Asai Y, Nakazawa A, Nakazawa T.
https://www.ncbi.nlm.nih.gov/pubmed/3011741

Engineering a d-lactate dehydrogenase that can super-efficiently utilize NADPH and NADH as cofactors.
Meng H1,2, Liu P3, Sun H2,3, Cai Z2, Zhou J2, Lin J3,4, Li Y2.
https://www.ncbi.nlm.nih.gov/pubmed/27109778