Difference between revisions of "Part:BBa K2151000:Design"

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===Design Notes===
 
===Design Notes===
  
−
The sequence was not altered for use in <i>E. coli</i> as the aim was to achieve expression in <i>S. thermophilus</i>
+
The sequence was not altered for use in <i>E. coli</i> as the aim was to achieve expression in <i>S. thermophilus</i>.
  
 
===Source===
 
===Source===
  
 
<ul>
 
<ul>
−
<li>Sequence obtained from 'Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene'.</li>
+
<li>Sequence obtained from 'Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene'.</li>
  
 
<li>Synthesized by IDT as an oligo-nucleotide</li>
 
<li>Synthesized by IDT as an oligo-nucleotide</li>

Latest revision as of 20:55, 8 October 2016


pHLBA, strong constitutive S. thermophilus promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was not altered for use in E. coli as the aim was to achieve expression in S. thermophilus.

Source

  • Sequence obtained from 'Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene'.
  • Synthesized by IDT as an oligo-nucleotide

References

Chouayekh, H., Serror, P., Boudebbouze, S. and Maguin, E. (2009) 'Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene', Fems Microbiology Letters, 293(2), pp. 232-239.