Difference between revisions of "Part:BBa K2088008"

Latest revision as of 10:49, 15 October 2016

catechol 2,3-dioxygenase gene with native rbs

This part includes catechol 2,3-dioxygenase(C23O) gene from Sphingobium sp. YBL2 and its native ribosome binding site. With a promoter before them, this gene can be used to degrade catechol, yeilding bright yellow product 2-hydroxymuconate semialdehyde with an absorbance maximum around 375 nm. As a result, this part can also be utilized as a reporter. As for the gene expression result please visit part BBa_K2088009.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 839

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 <partinfo>BBa_K2088008 short</partinfo>
-This part includes catechol 2,3-dioxygenase(C23O) gene from Sphingobium sp. YBL2 and its native ribosome binding site. With a promoter before them, this gene can be used to degrade catechol, yeilding bright yellow product 2-hydroxymuconate semialdehyde with an absorbance maximum around 375 nm. As a result, this part can also be utilized as a reporter.
+This part includes catechol 2,3-dioxygenase(C23O) gene from Sphingobium sp. YBL2 and its native ribosome binding site. With a promoter before them, this gene can be used to degrade catechol, yeilding bright yellow product 2-hydroxymuconate semialdehyde with an absorbance maximum around 375 nm. As a result, this part can also be utilized as a reporter. As for the gene expression result please visit part BBa_K2088009.
 <!-- Add more about the biology of this part here