Difference between revisions of "Part:BBa K2066028:Experience"

Latest revision as of 03:18, 29 October 2016

William and Mary iGEM 2016 sequence confirmed this part.

We also validated its fluorescence, induction capability, and repressor functionality (in combination with a pLac GFP reporter BBa_K2066014) through flow cytometry.

Validation of fluorescence and induction capability:

We did not obtain plots for repressor functionality due to a data storage error, but we did confirm that with fixed Arabinose concentration at 1 mM and variable IPTG induction over 0 - 10 mM range, that FL3 fluorescence levels (to detect mCherry) were were constant across the induction (in arbitrary fluorescence units) while the FL1 fluorescence levels (to detect sfGFP from BBa_K2066014) increased with induction (in arbitrary fluorescence units).

Applications of BBa_K2066028

User Reviews

UNIQ7173cf52864085d8-partinfo-00000000-QINU UNIQ7173cf52864085d8-partinfo-00000001-QINU

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 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+William and Mary iGEM 2016 sequence confirmed this part.
-how you used this part and how it worked out.
+We also validated its fluorescence, induction capability, and repressor functionality (in combination with a pLac GFP reporter BBa_K2066014) through flow cytometry.
+Validation of fluorescence and induction capability:
+https://static.igem.org/mediawiki/parts/9/97/William_and_Mary_28_IC3_Induction.png
+We did not obtain plots for repressor functionality due to a data storage error, but we did confirm that with fixed Arabinose concentration at 1 mM and variable IPTG induction over 0 - 10 mM range, that FL3 fluorescence levels (to detect mCherry) were were constant across the induction (in arbitrary fluorescence units) while the FL1 fluorescence levels (to detect sfGFP from BBa_K2066014) increased with induction (in arbitrary fluorescence units).
 ===Applications of BBa_K2066028===