Difference between revisions of "Part:BBa K2110013"
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
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<partinfo>BBa_K2110013 SequenceAndFeatures</partinfo>
  

Latest revision as of 17:08, 13 October 2016


PETase enzyme after codon optimization for bacillus subtilis

This gene encodes thePETase enzyme. This protein can bind on thePET surface and degrade PET to MHET, which have higher efficiency than other cutinase. At first, this gene couldn’t fully express in bacillus subtilis. So we use codon optimizationfor bacillus subtilis for better expression and secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 639
  • 1000
    COMPATIBLE WITH RFC[1000]