Difference between revisions of "Part:BBa K2020001"

Latest revision as of 20:17, 8 October 2016

subtilisin E gene with leader sequence pelB

This DNA sequence can be used to express subtilisin E in Escherichia coli and simultaneously secret the enzyme into the periplasm of the cell.
This composite part consists of the leader sequence pelB (BBa_J32015) and the subtilisin E gene (BBa_K2020000).

Usage and Biology

Subtilisin E is an alkaline serine protease which non-specifically digests proteins. It is naturally produced by Bacillus subtilis.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 54
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2020001 short</partinfo>
-This composite part consists of the leader sequence pelB ([[Part:BBa_J32015|BBa_J32015]]) and the subtilisin E gene ([[Part:BBa_K2020000|BBa_K2020000]]). It can be used to express subtilisin E in ''Escherichia coli'' and simultaneously secret the enzyme into the periplasm of the cell. Subtilisin E is an alkaline serine protease which non-specifically digests proteins.
+This DNA sequence can be used to express subtilisin E in ''Escherichia coli'' and simultaneously secret the enzyme into the periplasm of the cell. <br>
+This composite part consists of the leader sequence pelB ([[Part:BBa_J32015|BBa_J32015]]) and the subtilisin E gene ([[Part:BBa_K2020000|BBa_K2020000]]).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
+Subtilisin E is an alkaline serine protease which non-specifically digests proteins. It is naturally produced by ''Bacillus subtilis''.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K2020001 SequenceAndFeatures</partinfo>