Difference between revisions of "Part:BBa K1961004:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | This sequence consists of two sub-parts: (1) TetR generator under under control of PTet, which has TetR binding sites; (2) PbrR generator expressed by GFP under control of PTet. We used tetracycline to induce the transcription. In order to transcribe PbrR and GFP, we removed the stop codon at PbrR | |
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+ | [[File: HSiTAIWAN_WIKI_K1961004_auto_map.png|400px|thumb|left|alt text]] | ||
===Source=== | ===Source=== | ||
We use parts in the 2016 iGEM plate | We use parts in the 2016 iGEM plate | ||
+ | R0040, B0034, C0040, B0010, B0012, R0040, B0034, I721002, E0040, B0010, B0012 | ||
===References=== | ===References=== |
Latest revision as of 07:14, 23 October 2016
PbrR generator expressed by GFP under autoregulation of PTet
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2040
Design Notes
This sequence consists of two sub-parts: (1) TetR generator under under control of PTet, which has TetR binding sites; (2) PbrR generator expressed by GFP under control of PTet. We used tetracycline to induce the transcription. In order to transcribe PbrR and GFP, we removed the stop codon at PbrR
Source
We use parts in the 2016 iGEM plate R0040, B0034, C0040, B0010, B0012, R0040, B0034, I721002, E0040, B0010, B0012