Difference between revisions of "Part:BBa K2036032:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | CII | + | CII can serve as transcriptional activator to initiate pRE. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | CII and pRE are both from bacteriophage lambda. | |
| − | CII | + | |
===References=== | ===References=== | ||
Marr M T, Roberts J W, Brown S E, et al. Interactions among CII protein, RNA polymerase and the λ PRE promoter: contacts between RNA polymerase and the–35 region of PRE are identical in the presence and absence of CII protein[J]. Nucleic acids research, 2004, 32(3): 1083-1090. | Marr M T, Roberts J W, Brown S E, et al. Interactions among CII protein, RNA polymerase and the λ PRE promoter: contacts between RNA polymerase and the–35 region of PRE are identical in the presence and absence of CII protein[J]. Nucleic acids research, 2004, 32(3): 1083-1090. | ||
Latest revision as of 09:14, 3 October 2016
CII-TT-pRE-RBS-GFP-LVAssrAtag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1142
Design Notes
CII can serve as transcriptional activator to initiate pRE.
Source
CII and pRE are both from bacteriophage lambda.
References
Marr M T, Roberts J W, Brown S E, et al. Interactions among CII protein, RNA polymerase and the λ PRE promoter: contacts between RNA polymerase and the–35 region of PRE are identical in the presence and absence of CII protein[J]. Nucleic acids research, 2004, 32(3): 1083-1090.