Difference between revisions of "Part:BBa K2092005"
 
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<partinfo>BBa_K2092005 short</partinfo>
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<partinfo>BBa_K2092004 short</partinfo>
  
 
This part encodes for the <i>alcR</i> gene, which is a positive regulatory gene in the ethanol regulon of filamentous fungus <i>Aspergillus nidulans (A.nidulans)</i>. It encodes the AlcR protein, an ethanol-activated transcription factor which would bind to its target promoters, including the alcA <a href="https://parts.igem.org/Part:BBa_K2092002">(PalcA)</a>  and aldA promoters.
 
This part encodes for the <i>alcR</i> gene, which is a positive regulatory gene in the ethanol regulon of filamentous fungus <i>Aspergillus nidulans (A.nidulans)</i>. It encodes the AlcR protein, an ethanol-activated transcription factor which would bind to its target promoters, including the alcA <a href="https://parts.igem.org/Part:BBa_K2092002">(PalcA)</a>  and aldA promoters.
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AlcR is also known as a zinc binuclear cluster activator as it contains a DNA-binding domain belonging to the C6 zinc binuclear cluster family. AlcR is unique in that it can bind to symmetric and asymmetric DNA sites with the same apparent affinity and also bind to single site with high affinity [1].
 
AlcR is also known as a zinc binuclear cluster activator as it contains a DNA-binding domain belonging to the C6 zinc binuclear cluster family. AlcR is unique in that it can bind to symmetric and asymmetric DNA sites with the same apparent affinity and also bind to single site with high affinity [1].
  
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A strong RBS <a href="https://parts.igem.org/Part:BBa_J34801">Part BBa_J34801</a> has been added upstream of the start codon.
  
  
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Reference:
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===Usage and Biology===
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This part is useful as an ethanol reporter.
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===Reference===
  
 
[1] Felenbok, B., Sequeval, D., Mathieu, M., Sibley, S., Gwynne, D.I. and Davies, R.W. (1988). The ethanol regulon in Aspergillus nidulans: Characterization and sequence of the positive regulatory gene alcR. <i>Gene</i>, 73(2), 385–396.
 
[1] Felenbok, B., Sequeval, D., Mathieu, M., Sibley, S., Gwynne, D.I. and Davies, R.W. (1988). The ethanol regulon in Aspergillus nidulans: Characterization and sequence of the positive regulatory gene alcR. <i>Gene</i>, 73(2), 385–396.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2092005 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2092004 SequenceAndFeatures</partinfo>
  
  
 
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<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2092005 parameters</partinfo>
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<partinfo>BBa_K2092004 parameters</partinfo>
 
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Latest revision as of 22:36, 2 October 2016

alcR (incl RBS), ethanol-activated transcription factor from A. nidulans

This part encodes for the alcR gene, which is a positive regulatory gene in the ethanol regulon of filamentous fungus Aspergillus nidulans (A.nidulans). It encodes the AlcR protein, an ethanol-activated transcription factor which would bind to its target promoters, including the alcA <a href="https://parts.igem.org/Part:BBa_K2092002">(PalcA)</a> and aldA promoters.

AlcR is also known as a zinc binuclear cluster activator as it contains a DNA-binding domain belonging to the C6 zinc binuclear cluster family. AlcR is unique in that it can bind to symmetric and asymmetric DNA sites with the same apparent affinity and also bind to single site with high affinity [1].

A strong RBS <a href="https://parts.igem.org/Part:BBa_J34801">Part BBa_J34801</a> has been added upstream of the start codon.


Usage and Biology

This part is useful as an ethanol reporter.


Reference

[1] Felenbok, B., Sequeval, D., Mathieu, M., Sibley, S., Gwynne, D.I. and Davies, R.W. (1988). The ethanol regulon in Aspergillus nidulans: Characterization and sequence of the positive regulatory gene alcR. Gene, 73(2), 385–396.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2568
    Illegal BamHI site found at 1572
    Illegal BamHI site found at 2310
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1276
    Illegal AgeI site found at 1404
    Illegal AgeI site found at 1935
  • 1000
    COMPATIBLE WITH RFC[1000]