Difference between revisions of "Part:BBa K2060001"
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<partinfo>BBa_K2060001 short</partinfo> | <partinfo>BBa_K2060001 short</partinfo> | ||
| − | mKeima is a red-shifted fluorescent protein with Excitation of 440nM and Emission of | + | mKeima is a red-shifted fluorescent protein with Excitation of 440nM and Emission of 620nM. In this part mKeima expression is induced by Arabinose driven by the wildtype [https://parts.igem.org/Part:BBa_I13453 pBAD promotor]. The [https://www.ncbi.nlm.nih.gov/protein/AMO27260 red shifted emission spectrum] gives it good potential for mammalian cell or tissue bioimaging. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | We attempted to use arabinose to induce expression of this biobrick variety of concentrations and time periods. Our success with this expression are documented in the [https://parts.igem.org/Part:BBa_K2060001:Experience Experience] section. | ||
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Latest revision as of 12:01, 19 October 2016
Fluorescent protein mKeima expressed by Arabinose promotor
mKeima is a red-shifted fluorescent protein with Excitation of 440nM and Emission of 620nM. In this part mKeima expression is induced by Arabinose driven by the wildtype pBAD promotor. The red shifted emission spectrum gives it good potential for mammalian cell or tissue bioimaging.
Usage and Biology
We attempted to use arabinose to induce expression of this biobrick variety of concentrations and time periods. Our success with this expression are documented in the Experience section.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]