Difference between revisions of "Part:BBa K2042008:Design"

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===Design Notes===
 
===Design Notes===
−
This enzyme is for increasing lactate
+
The sequence was codon optimized for Pseudomonas putida, three amino acid mutation were introduce (D176S, I177R, F178T) to efficiently use both NADH and NADPH as cofactors. Silent mutation were also introduced for removing restriction site.
  
 
===Source===
 
===Source===

Latest revision as of 20:21, 1 October 2016


Engineered D-Lactate dehydrogenase (D-LDH*)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 630
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for Pseudomonas putida, three amino acid mutation were introduce (D176S, I177R, F178T) to efficiently use both NADH and NADPH as cofactors. Silent mutation were also introduced for removing restriction site.

Source

Synthetic synthesis

References