Difference between revisions of "Part:BBa J100305:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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We grew <i>E. coli </i>cells with three different experimental groups; negative control should be green, positive control should be red, and UPP promoter. We took a colony from each experimental group, with two colonies from UPP, and grew one of each with UTP, which should have activated the promoter. This graph contains the averages of 3 samples from each experimental group with and without UTP. We measured the fluorescence and absorbance of each sample using a spectrophotometer and calculated the relative fluorescence intensity by finding the ratio between the fluorescence and absorbance (cell density).  
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how you used this part and how it worked out.
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[[File:UPP_RFP.png|500px|]]
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[[File:UPP_GFP.png|500px|]]
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===Applications of BBa_J100305===
 
===Applications of BBa_J100305===

Latest revision as of 19:27, 22 September 2016


We grew E. coli cells with three different experimental groups; negative control should be green, positive control should be red, and UPP promoter. We took a colony from each experimental group, with two colonies from UPP, and grew one of each with UTP, which should have activated the promoter. This graph contains the averages of 3 samples from each experimental group with and without UTP. We measured the fluorescence and absorbance of each sample using a spectrophotometer and calculated the relative fluorescence intensity by finding the ratio between the fluorescence and absorbance (cell density).

UPP RFP.png


UPP GFP.png


Applications of BBa_J100305

User Reviews

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