Difference between revisions of "Part:BBa K2027001:Experience"

 
(Applications of BBa_K2027001)
 
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===Applications of BBa_K2027001===
 
===Applications of BBa_K2027001===
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We used this part as a paper based biological thermometer to sense temperature changes by color change. Once this construct was successfully transformed and expressed in bacteria, the protein was extracted and dialyzed. The concentrated protein was then pipetted onto cellulose sheets and underwent a series of heat tests. It was found that, while this protein initially appeared blue, it begins to turn purple at 55 °C and this purple color becomes more pronounced as temperature is increased. Interestingly, when allowed to cool back to room temperature, the blue color returned.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 06:28, 24 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2027001

We used this part as a paper based biological thermometer to sense temperature changes by color change. Once this construct was successfully transformed and expressed in bacteria, the protein was extracted and dialyzed. The concentrated protein was then pipetted onto cellulose sheets and underwent a series of heat tests. It was found that, while this protein initially appeared blue, it begins to turn purple at 55 °C and this purple color becomes more pronounced as temperature is increased. Interestingly, when allowed to cool back to room temperature, the blue color returned.

User Reviews

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