Difference between revisions of "Part:BBa K2042011"

 
 
(3 intermediate revisions by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2042011 short</partinfo>
 
<partinfo>BBa_K2042011 short</partinfo>
  
a
+
This is the sequence of a P.putida ribosom binding site followed by a evolved Pseudomonas resinovorans PHA synthase 1 gene. This translational unit produce a enzyme which take monomer of (D)-lactyl-CoA and produce PLA homopolymer. It was used in the part:
 +
<html>
 +
<a href="https://parts.igem.org/Part:BBa_K2042013" title="BBa_K2042013">BBa_K2042013</a><br>
 +
</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 9: Line 12:
  
 
<!-- -->
 
<!-- -->
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<partinfo>BBa_K2042011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2042011 SequenceAndFeatures</partinfo>
  

Latest revision as of 21:44, 15 October 2016


RBS +PhaC_resinovorans

This is the sequence of a P.putida ribosom binding site followed by a evolved Pseudomonas resinovorans PHA synthase 1 gene. This translational unit produce a enzyme which take monomer of (D)-lactyl-CoA and produce PLA homopolymer. It was used in the part: BBa_K2042013

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 100
    Illegal XhoI site found at 185
    Illegal XhoI site found at 1043
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1073
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 809