Difference between revisions of "Part:BBa K2066014:Design"

 
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__NOTOC__
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<partinfo>BBa_K2066014 short</partinfo>
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<partinfo>BBa_K2066014 SequenceAndFeatures</partinfo>
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===Design Notes===
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Part designed to be compared with K2066026 (without RiboJ) to see the effect of RiboJ on the transfer function.
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===Source===
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This part was created using IDT DNA Synthesis.
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The UNS2 and UNS3 sequences are taken from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”). RiboJ sequence is from Lou et. al 2012 ("Ribozyme-based insulator parts buffer synthetic circuits from genetic context").
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===References===

Latest revision as of 00:33, 20 October 2016


plLacO-1+RiboJ+sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 224


Design Notes

Part designed to be compared with K2066026 (without RiboJ) to see the effect of RiboJ on the transfer function.

Source

This part was created using IDT DNA Synthesis.

The UNS2 and UNS3 sequences are taken from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”). RiboJ sequence is from Lou et. al 2012 ("Ribozyme-based insulator parts buffer synthetic circuits from genetic context").

References