Difference between revisions of "Part:BBa K2066053:Design"

 
(→‎=Design Notes)
 
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===Design Notes===
 
===Design Notes===
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Made this part to make multiple plasmid transformations more effective.  
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The part was made to put K2066022 and K2066023 on the same plasmid.  
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===Source===
 
===Source===
  
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This part was derived mostly from iGEM registry parts.  It is a combination of K2066022 and K2066023.  It uses UNS sequences from Torella et. al 2013 ("Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly")  
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This part was derived mostly from iGEM registry parts.  It is a combination of K2066022 and K2066023.  It uses UNS sequences from Torella et. al 2013 ("Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly")
  
 
===References===
 
===References===

Latest revision as of 02:28, 20 October 2016


pTET GFP + TetR on UNS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1004
    Illegal NheI site found at 1027
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 764


Design Notes

The part was made to put K2066022 and K2066023 on the same plasmid.


Source

This part was derived mostly from iGEM registry parts. It is a combination of K2066022 and K2066023. It uses UNS sequences from Torella et. al 2013 ("Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly")

References