Difference between revisions of "Part:BBa K2151008"

 
 
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<partinfo>BBa_K2151008 short</partinfo>
 
<partinfo>BBa_K2151008 short</partinfo>
  
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<p>This part consists of I13500 (GFP with strong ribosome binding site) under the control of p32, a strong constitutive bacterial promoter. Its intended use is to serve as a promoter-reporter construct in lactic acid bacteria. p32 is used as a promoter in expression vectors functioning within <i>Streptococcus thermophilus</i> </p>
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<p>We used p32 as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of p32 using GFP revealed promoter strength on par with strong Anderson promoters (81% as strong as J23100, 115% as strong as J23101).</p>
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[[File:T--Glasgow--B0034 Measurement.jpg]]
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<p><b>Figure 1: </b>Mean fluorescence values for I13500 under the control of various promoters. Orange represents <i>S. thermophilus</i> promoters, blue represents Anderson family promoters. Error bars represent standard deviation for each set of samples.</p>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 05:00, 29 October 2016


p32-I13500: GFP under control of strong constitutive promoter and strong ribosome binding site

This part consists of I13500 (GFP with strong ribosome binding site) under the control of p32, a strong constitutive bacterial promoter. Its intended use is to serve as a promoter-reporter construct in lactic acid bacteria. p32 is used as a promoter in expression vectors functioning within Streptococcus thermophilus

We used p32 as a candidate promoter to drive gene expression in both Streptococcus thermophilus and E. coli. Quantification of p32 using GFP revealed promoter strength on par with strong Anderson promoters (81% as strong as J23100, 115% as strong as J23101).

T--Glasgow--B0034 Measurement.jpg

Figure 1: Mean fluorescence values for I13500 under the control of various promoters. Orange represents S. thermophilus promoters, blue represents Anderson family promoters. Error bars represent standard deviation for each set of samples.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 720