Difference between revisions of "Part:BBa K2151004"

Latest revision as of 05:04, 29 October 2016

p25-E5501: GFP under control of medium constitutive promoter

This part consists of E5501 (GFP with medium ribosome binding site) under the control of p25, a medium constitutive bacterial promoter. Its intended use is to serve as a promoter-reporter construct in lactic acid bacteria. p25 was initially identified in a promoter probe survey of the Streptococcus thermophilus genome, alongside weaker promoters p8 and p20.

We used p25 as a candidate promoter to drive gene expression in both Streptococcus thermophilus and E. coli. Quantification of p25 using GFP in E. coli revealed promoter strength on par with medium Anderson promoters (30.8% as strong as J23100, 43.7% as strong as J23101, 130% as strong as J23106).

Figure 2: Mean fluorescence values for E5501 under the control of various promoters. Red represents S. thermophilus promoters, blue represents Anderson family promoters. Error bars represent standard deviation for each set of samples. Promoters are ordered according to their strength when expressing I13500.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 708

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 <partinfo>BBa_K2151004 short</partinfo>
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+<p>This part consists of E5501 (GFP with medium ribosome binding site) under the control of p25, a medium constitutive bacterial promoter. Its intended use is to serve as a promoter-reporter construct in lactic acid bacteria. p25 was initially identified in a promoter probe survey of the <i>Streptococcus thermophilus</i> genome, alongside weaker promoters p8 and p20.</p>
+ <p>We used p25 as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of p25 using GFP in <i>E. coli</i> revealed promoter strength on par with medium Anderson promoters (30.8% as strong as J23100, 43.7% as strong as J23101, 130% as strong as J23106).</p>
+[[File:T--Glasgow--B0032 Measurement.jpg]]
+<b>Figure 2:</b> Mean fluorescence values for E5501 under the control of various promoters. Red represents S. thermophilus promoters, blue represents Anderson family promoters. Error bars represent standard deviation for each set of samples. Promoters are ordered according to their strength when expressing I13500.
 <!-- Add more about the biology of this part here