Difference between revisions of "Part:BBa K2066022"
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This is a composite part with a constitutive Anderson promoter J23105 to express TetR repressor. Part used to express the repressor TetR that can bind to pTET (promoter) to block the expression of GFP (or any gene that pTET controls). The tetR repressor protein can be temporarily blocked by aTc induction to allow for transcription of gene product controlled by pTET. | This is a composite part with a constitutive Anderson promoter J23105 to express TetR repressor. Part used to express the repressor TetR that can bind to pTET (promoter) to block the expression of GFP (or any gene that pTET controls). The tetR repressor protein can be temporarily blocked by aTc induction to allow for transcription of gene product controlled by pTET. | ||
+ | |||
+ | This part is on the UNS backbone standard developed by William and Mary iGEM 2016, for ease of cloning via Gibson or Golden Gate Assembly. The insert is flanked by UNS 2 (BBa_K2066018) and UNS 3 (BBa_K2066019) sequences. | ||
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Latest revision as of 02:01, 29 October 2016
Constitutive tetR + UNS
This is a composite part with a constitutive Anderson promoter J23105 to express TetR repressor. Part used to express the repressor TetR that can bind to pTET (promoter) to block the expression of GFP (or any gene that pTET controls). The tetR repressor protein can be temporarily blocked by aTc induction to allow for transcription of gene product controlled by pTET.
This part is on the UNS backbone standard developed by William and Mary iGEM 2016, for ease of cloning via Gibson or Golden Gate Assembly. The insert is flanked by UNS 2 (BBa_K2066018) and UNS 3 (BBa_K2066019) sequences.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 47
Illegal NheI site found at 70 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]