Difference between revisions of "Part:BBa K2042000:Design"

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===Design Notes===
 
===Design Notes===
−
The iGEM Team Evry 2016 used this enzyme as the first enzyme in the pathway to create Polylactic acid (PLA).
+
This is the DNA coding for the enzyme propionate CoA transferase from Clostridum propionicum. The sequence was codon optimized for Pseudomonas putida and one amino acid mutation (A243T) was introduce in order to produce (D)-Lactyl-CoA.
 +
For the design, silent mutation was used for removing restriction site.
 +
The iGEM Team Evry 2016 used this enzyme as the first enzyme in the pathway to produce Polylactic acid (PLA) in Pseudomonas putida.
  
 
===Source===
 
===Source===
  
−
Synthetic synthesis
+
This sequence was synthetized by IDT
  
 
===References===
 
===References===
−
Biosynthesis of polylactic acid and its copolymers using evolved propionate CoA transferase and PHA synthase.
+
Biosynthesis of Polylactic Acid and Its Copolymers using Evolved Propionate CoA Transferase and PHA Synthase <br>
−
Yang TH1, Kim TW, Kang HO, Lee SH, Lee EJ, Lim SC, Oh SO, Song AJ, Park SJ, Lee SY.
+
Taek Ho Yang,1 Tae Wan Kim,1 Hye Ok Kang,1 Sang-Hyun Lee,1 Eun Jeong Lee,1 Sung-Chul Lim,1 Sun Ok Oh,1 Ae-Jin Song,2 Si Jae Park,1 Sang Yup Lee2,3 <br>
 +
http://www.ncbi.nlm.nih.gov/pubmed/19937726
  
−
Tailor-made type II Pseudomonas PHA synthases and their use for the biosynthesis of polylactic acid and its copolymer in recombinant Escherichia coli.
+
Microbial production of lactate-containing polyesters. <br>
−
Yang TH1, Jung YK, Kang HO, Kim TW, Park SJ, Lee SY.
+
Yang JE1, Choi SY, Shin JH, Park SJ, Lee SY.<br>
 +
http://www.ncbi.nlm.nih.gov/pubmed/23718266

Latest revision as of 17:20, 20 September 2016


Propionate CoA transferase for P. putida


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1081
    Illegal BsaI site found at 1552


Design Notes

This is the DNA coding for the enzyme propionate CoA transferase from Clostridum propionicum. The sequence was codon optimized for Pseudomonas putida and one amino acid mutation (A243T) was introduce in order to produce (D)-Lactyl-CoA. For the design, silent mutation was used for removing restriction site. The iGEM Team Evry 2016 used this enzyme as the first enzyme in the pathway to produce Polylactic acid (PLA) in Pseudomonas putida.

Source

This sequence was synthetized by IDT

References

Biosynthesis of Polylactic Acid and Its Copolymers using Evolved Propionate CoA Transferase and PHA Synthase
Taek Ho Yang,1 Tae Wan Kim,1 Hye Ok Kang,1 Sang-Hyun Lee,1 Eun Jeong Lee,1 Sung-Chul Lim,1 Sun Ok Oh,1 Ae-Jin Song,2 Si Jae Park,1 Sang Yup Lee2,3
http://www.ncbi.nlm.nih.gov/pubmed/19937726

Microbial production of lactate-containing polyesters.
Yang JE1, Choi SY, Shin JH, Park SJ, Lee SY.
http://www.ncbi.nlm.nih.gov/pubmed/23718266