Difference between revisions of "Part:BBa K1943005:Design"

 
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===Design Notes===
 
===Design Notes===
−
no
+
This biobrick is constructed through 2 steps:
−
 
+
  
 +
# Do double enzyme digestion (EcoRI & SpeI for BBa_K592012, EcoRI & XbaI for BBa_B0015) and do gel extraction, ligation, transformation, picking up single colonies, LB broth incubation, plasmid extraction and gel electrophoresis verification.
 +
# Do double enzyme digestion (XbaI & PstI for Step 1 product, SpeI & PstI for BBa_K608007) and do gel extraction, ligation, transformation, picking up single colonies, LB broth incubation, plasmid extraction and gel electrophoresis verification.
  
 
===Source===
 
===Source===
 +
The following list is the Biobricks we’ve used in construction and their detailed design information.
  
−
basic parts
+
{| class="wikitable"
 +
! Part
 +
! Type
 +
! Discription
 +
! Backbone
 +
! Location on the plate
 +
|-
 +
| BBa_K880005
 +
| Promoter + RBS
 +
| Strong Promoter + Strong RBS
 +
| pSB1C3
 +
| 3F 2016 Kit Plate 2
 +
|-
 +
| BBa_K608007
 +
| Promoter + RBS
 +
| Medium Promoter + Weak RBS
 +
| pSB1C3
 +
| 5G 2016 Kit Plate 1
 +
|-
 +
| BBa_K592012
 +
| Chromoprotein
 +
| eforRed, red chromoprotein
 +
| pSB1C3
 +
| 15I 2016 Kit Plate 6
 +
|-
 +
| BBa_K1033919
 +
| Chromoprotein
 +
| gfasPurple, purplr chromoprotein
 +
| pSB1C3
 +
| 9K 2016 Kit Plate 6
 +
|-
 +
| BBa_K1033910
 +
| Chromoprotein
 +
| fwYellow, yellow chromoprotein
 +
| pSB1C3
 +
| 6K 2016 Kit Plate 4
 +
|-
 +
| BBa_K1033932
 +
| Chromoprotein
 +
| spisPink, pink chromoprotein
 +
| pSB1C3
 +
| 11K 2016 Kit Plate 6
 +
|-
 +
| BBa_B0015
 +
| Terminator
 +
| Double terminator
 +
| pSB1C3
 +
| 3F 2016 Kit Plate 3
 +
|}
  
 
===References===
 
===References===
 +
#[[About_Assembly|Parts Registry Assembly Help]]
 +
#[[Help:Protocols/Transformation|Parts Registry Transformation Guideline]]

Latest revision as of 13:28, 16 October 2016


eforRed, Red chromoprotein reporter system (medium Promoter , weak RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick is constructed through 2 steps:

  1. Do double enzyme digestion (EcoRI & SpeI for BBa_K592012, EcoRI & XbaI for BBa_B0015) and do gel extraction, ligation, transformation, picking up single colonies, LB broth incubation, plasmid extraction and gel electrophoresis verification.
  2. Do double enzyme digestion (XbaI & PstI for Step 1 product, SpeI & PstI for BBa_K608007) and do gel extraction, ligation, transformation, picking up single colonies, LB broth incubation, plasmid extraction and gel electrophoresis verification.

Source

The following list is the Biobricks we’ve used in construction and their detailed design information.

Part Type Discription Backbone Location on the plate
BBa_K880005 Promoter + RBS Strong Promoter + Strong RBS pSB1C3 3F 2016 Kit Plate 2
BBa_K608007 Promoter + RBS Medium Promoter + Weak RBS pSB1C3 5G 2016 Kit Plate 1
BBa_K592012 Chromoprotein eforRed, red chromoprotein pSB1C3 15I 2016 Kit Plate 6
BBa_K1033919 Chromoprotein gfasPurple, purplr chromoprotein pSB1C3 9K 2016 Kit Plate 6
BBa_K1033910 Chromoprotein fwYellow, yellow chromoprotein pSB1C3 6K 2016 Kit Plate 4
BBa_K1033932 Chromoprotein spisPink, pink chromoprotein pSB1C3 11K 2016 Kit Plate 6
BBa_B0015 Terminator Double terminator pSB1C3 3F 2016 Kit Plate 3

References

  1. Parts Registry Assembly Help
  2. Parts Registry Transformation Guideline