Difference between revisions of "Part:BBa K2180011"

 
 
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<partinfo>BBa_K2180011 short</partinfo>
 
<partinfo>BBa_K2180011 short</partinfo>
  
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We add two restriction sites. SacII before and PacI after the regulatory region in order to use it in our project. They allow us to replace a regulatory region with another regulatory region. This Region allow ArsR to bind to DNA and repress transcription. In the presence of Heavy Metals such as Arsenic or Cadmium transcription is derepressed because of the fixation to ArsR.
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Biobrick based on a previous biobrick made by the iGEM09_Newcastle Team (2009-10-14) :
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Other metalic ions may bind to ArsR.
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<br>https://parts.igem.org/Part:BBa_K174015
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<br>We added two restriction sites: SacII before and PacI after the regulatory region in order to use it in our project. They allow us to replace a regulatory region with another regulatory region. This Region allows ArsR to bind to DNA and repress transcription. In the presence of Heavy Metals such as Arsenic or Cadmium transcription is derepressed because of the fixation to ArsR.
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<br>Other metalic ions may bind to ArsR.
  
 
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[[File:T--UPMC-Paris--Seq4b.png|600px]]<br>
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The insert can also be confirmed by digestion using a unique restriction site inside the biobrick and checked by electrophoresis migration<br><br>
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2180011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2180011 SequenceAndFeatures</partinfo>

Latest revision as of 06:39, 30 October 2016


Arsenic Operator - ArsR Binding Site

Biobrick based on a previous biobrick made by the iGEM09_Newcastle Team (2009-10-14) :
https://parts.igem.org/Part:BBa_K174015
We added two restriction sites: SacII before and PacI after the regulatory region in order to use it in our project. They allow us to replace a regulatory region with another regulatory region. This Region allows ArsR to bind to DNA and repress transcription. In the presence of Heavy Metals such as Arsenic or Cadmium transcription is derepressed because of the fixation to ArsR.
Other metalic ions may bind to ArsR.

T--UPMC-Paris--Seq4b.png
The insert can also be confirmed by digestion using a unique restriction site inside the biobrick and checked by electrophoresis migration

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]