Difference between revisions of "Part:BBa K2033001"
 
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<partinfo>BBa_K2033001 short</partinfo>
 
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===Short Description===
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This part responds to the HSL quorum sensing molecule N-dodecanoyl-L-homoserine lactone (C(12)-HSL). This is HSL is produced by AubI (Part:''<partinfo>C0062</partinfo>'')
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<partinfo>BBa_K2033001 parameters</partinfo>
 
<partinfo>BBa_K2033001 parameters</partinfo>
 
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===Short Description===
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This part responds to the HSL quorum sensing molecule N-dodecanoyl-L-homoserine lactone (C(12)-HSL). This is HSL is produced by AubI (Part:''<partinfo>K2033000</partinfo>'')
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===Introduction to HSL Quorum Sensing===
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HSLs, or Homoserine Lactones, are a common chemical compound produced by a wide range of bacteria to communicate. As a major variant of quorum sensing, "N-Acyl homoserine lactones" come in many forms, although they share the same basic backbone shown below:
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<div style="text-align: center;">[[File:T--Arizona State--HSLMolecule.jpg]]</div>
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Distinguishable by its lactone ring, HSLs in quorum sensing are used as a density-dependent communication system for many bacteria that controls growth rate, virulence, and bio-luminescence among other things. The Aub system originates from an unidentified soil bacteria and is highly uncharacterized.
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HSL quorum sensing functions within two modules. The first module, the "Sender," must be induced by certain environmental conditions, usually population density of surrounding organisms. This will begin production of the HSL by the cell, which is then detected by the second module, the "Receiver." Once a certain threshold of HSLs is breached, the Receiver will cause the expression or silencing of certain genes to achieve the desired purpose of the communication, whether it is the production of GFP or to increase growth rate.

Latest revision as of 06:20, 19 September 2016

C(12)-HSL Receiver Device - AubR


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 395
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 395
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 395
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 395
    Illegal AgeI site found at 205
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 184


Short Description

This part responds to the HSL quorum sensing molecule N-dodecanoyl-L-homoserine lactone (C(12)-HSL). This is HSL is produced by AubI (Part:BBa_K2033000)

Introduction to HSL Quorum Sensing

HSLs, or Homoserine Lactones, are a common chemical compound produced by a wide range of bacteria to communicate. As a major variant of quorum sensing, "N-Acyl homoserine lactones" come in many forms, although they share the same basic backbone shown below:

T--Arizona State--HSLMolecule.jpg

Distinguishable by its lactone ring, HSLs in quorum sensing are used as a density-dependent communication system for many bacteria that controls growth rate, virulence, and bio-luminescence among other things. The Aub system originates from an unidentified soil bacteria and is highly uncharacterized.

HSL quorum sensing functions within two modules. The first module, the "Sender," must be induced by certain environmental conditions, usually population density of surrounding organisms. This will begin production of the HSL by the cell, which is then detected by the second module, the "Receiver." Once a certain threshold of HSLs is breached, the Receiver will cause the expression or silencing of certain genes to achieve the desired purpose of the communication, whether it is the production of GFP or to increase growth rate.