Difference between revisions of "Part:BBa K2180004"

 
 
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<partinfo>BBa_K2180004 short</partinfo>
 
<partinfo>BBa_K2180004 short</partinfo>
  
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Coding sequence of LacI protein + LVA (rapid degradation tail) wich improve the switch time and make it more sensitive.  
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This is the assembly of our basic parts BBa_K2180009 , BBa_K2180007 and BBa_K2180008.
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<br>The NcoI restriction site is overlapping parts BBa_K2180009 and BBa_K2180007 at the Start codon of LacI + LVA.
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<br>This part is an adaptation for B. subtilis of the biobrick BBa_K1163999 from iGEM13_Evry (2013-09-02):
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<br>This system allow the synthese of the protein Lac I in the absence of Iron.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:31, 23 October 2016


Promoter +FUR regulatory sequence + RBS + LacI-LVA + Terminator

This is the assembly of our basic parts BBa_K2180009 , BBa_K2180007 and BBa_K2180008.
The NcoI restriction site is overlapping parts BBa_K2180009 and BBa_K2180007 at the Start codon of LacI + LVA.
This part is an adaptation for B. subtilis of the biobrick BBa_K1163999 from iGEM13_Evry (2013-09-02):
This system allow the synthese of the protein Lac I in the absence of Iron.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1360
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 377
    Illegal AgeI site found at 1165
  • 1000
    COMPATIBLE WITH RFC[1000]