Difference between revisions of "Part:BBa K2066001:Design"
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===Design Notes=== | ===Design Notes=== | ||
We wanted to maintain a standard that allowed us an easy and consistent way to PCR and gibson clone our samples, which we did not find in the Prefix and Suffix. PCRs and Gibson assemblies using USN 2 and 3 primers have been very effective. | We wanted to maintain a standard that allowed us an easy and consistent way to PCR and gibson clone our samples, which we did not find in the Prefix and Suffix. PCRs and Gibson assemblies using USN 2 and 3 primers have been very effective. | ||
| + | |||
| + | We chose to use the eFarRed chromoprotein developed by Uppsala iGEM 2012 to have an easily-visible indicator of the presence of the construct. | ||
Latest revision as of 01:46, 29 October 2016
UNS Standard Adapter with Red Chromoprotein.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 47
Illegal NheI site found at 70 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We wanted to maintain a standard that allowed us an easy and consistent way to PCR and gibson clone our samples, which we did not find in the Prefix and Suffix. PCRs and Gibson assemblies using USN 2 and 3 primers have been very effective.
We chose to use the eFarRed chromoprotein developed by Uppsala iGEM 2012 to have an easily-visible indicator of the presence of the construct.
Source
The UNS2 and UNS3 sequences are taken from: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860. Other sequences are from the iGEM registry.