Difference between revisions of "Part:BBa J100279:Design"
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Since there was only a 6 base difference between BD18 C Dog and BD10 C Dog, the PCR was used to insert in and amplify the new BD10 C Dog RBS rather than during GGA. GGA was still necessary to make the dsDNA into a plasmid. | Since there was only a 6 base difference between BD18 C Dog and BD10 C Dog, the PCR was used to insert in and amplify the new BD10 C Dog RBS rather than during GGA. GGA was still necessary to make the dsDNA into a plasmid. | ||
| − | + | Note that the PDB riboswitch's last base is a C instead of a T, which may help this riboswitch to be less leaky. | |
===Source=== | ===Source=== | ||
Latest revision as of 14:47, 6 July 2016
tClone Tet+PDB riboswitch with P9 and BD10
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 350
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 496
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 522
Illegal NgoMIV site found at 890
Illegal NgoMIV site found at 1050 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Since there was only a 6 base difference between BD18 C Dog and BD10 C Dog, the PCR was used to insert in and amplify the new BD10 C Dog RBS rather than during GGA. GGA was still necessary to make the dsDNA into a plasmid.
Note that the PDB riboswitch's last base is a C instead of a T, which may help this riboswitch to be less leaky.
Source
J100275