Latest revision as of 20:39, 16 May 2016

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< RFC25 assembly standard description

RFC25 BioBrick parts

RFC25 compatible BioBrick backbones

For the cloning of RFC25 BioBricks into the Parts Registry compatible backbone the plasmid pSB1C3-RFP_Generator (BBa_K801100) can be used.

Fluorescent proteins

Fluorescent proteins (click for all RFC25 parts)
Protein	Color	BioBrick	Excitation [nm]	Emission [nm]	Quantum Yield
Cerulean	Cyan	BBa_I757009	433	475	0.62
eCFP	Cyan	BBa_K1159302	439	476	0.40
GFP	Green	BBa_K648013	484	507	0.60
GFP	Green	BBa_K863120	484	507	0.60
GFP	Green	BBa_K823039	484	507	0.60
sfGFP	Green	BBa_K1321337	485	510	0.65
Dronpa (photoactivatable)	GreenYellow	BBa_K1680006	503	518	0.85
mVenus	Yellow	BBa_I757008	515	528	0.57
sYFP2	Yellow	BBa_K1159301	514	527	0.61
mRFP	Red	BBa_K1351021	584	607	0.25
mRFP	Red	BBa_K1615089	584	607	0.25
mKate2	Red	BBa_K823029	588	633	0.4
Values for the fluorescent proteins were taken from [http://www.microscopyu.com/articles/livecellimaging/fpintro.html\| microscopyu.com].

Luciferases

Luciferases (click for all RFC25 parts)
Protein	BioBrick	Size [kDa]
Firefly luciferase	BBa_K525999	61
Renilla luciferase	not available yet	36
NanoLuc luciferase	BBa_K1159001	19
Gaussia luciferase	not available yet

Transmembrane domains

Optogenetic parts

Linker domains

Linker (click for all RFC25 parts)
Protein	Sequence	BioBrick	Length [residues]
Short Linker	(Gly-Gly-Ser-Gly)	BBa_K243004	4
Middle Linker	2x (Gly-Gly-Ser-Gly)	BBa_K243005	8
Long Linker	3x (Gly-Gly-Ser-Gly)	BBa_K243006	12

Catalytic proteins

Catalytic proteins (click for all RFC25 parts)
Erythromycin Esterase Type II	BBa_K1159000
Laccase	BBa_K1159002
Laccase TVEL5	BBa_K1615067
TEV Protease	BBa_K1159100
Thermonuclease	BBa_K1159105
Heroin Esterase	BBa_K1615045
CRE recombinase	BBa_K1680007
Monoamine Oxidase A	BBa_K1615022
Endo-β-Galactosidase	BBa_K1483001
α-Galactosidase	BBa_K1483002
Acetylgalactosamindase	BBa_K1483000
2-oxoglutarate oxygenase/decarboxylase	BBa_K1065000

Transcription regulators

Transcription regulators (click for all RFC25 parts)
VP16	BBa_K1150001

Split proteins

Split proteins (click for all RFC25 parts)
TEV Protease	BBa_K1159101	BBa_K1159102
SpyCatcher/SpyTag	BBa_K1159200	BBa_K1159201

Binding proteins

Binding proteins (click for all RFC25 parts)
Fluorescein binding Anticalin	BBa_K1159003
Digoxigenin binding Anticalin	BBa_K243003
HER2 binding DARPin	BBa_K404302
HER2 binding Affibody	BBa_K404302
Nickel Binding Protein	BBa_K1321009
Cellulose binding Domain	BBa_K863110

Protease cleavage sites

Protease cleavage sites (click for all RFC25 parts)
Name	Sequence	BioBrick
TEV cleavage site	G-ENLYFQS-G	BBa_K1159318

Affinity tags

Affinity tags (click for all RFC25 parts)
HA tag	BBa_K1150016
His₆-tag	BBa_I757013
SpyTag	BBa_K1159020
Strep-tag II as N-part	BBa_K549018
Strep-tag II	BBa_J18916
cMyc-tag	BBa_K823036
3x FLAG tag	BBa_K823034

@@ Line 1: / Line 1: @@
+<span style="background:#32CD32">'''[[Collections|< Back to BioBrick collection]]'''</span><br>
+<span style="background:#32CD32">'''[[Assembly_standard_25|< RFC25 assembly standard description]]'''</span><br>
 =RFC25 BioBrick parts=
+==RFC25 compatible BioBrick backbones==
+[[File:RFC25Backbone.png|thumb|right|300px|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K801100'>pSB1C3-RFP_Generator (BBa_K801100)</A></HTML>]]
+For the cloning of RFC25 BioBricks into the Parts Registry compatible backbone the plasmid <HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K801100'>pSB1C3-RFP_Generator (BBa_K801100)</A></HTML> can be used.<br>
+<br><br><br><br><br><br><br><br><br><br>
 ==Fluorescent proteins==
 {| class="wikitable"
-!colspan="6"|Fluorescent proteins
+!colspan="6"|[[RFC25_"Freiburg_standard"_parts#Fluorescent_proteins| Fluorescent proteins (click for all RFC25 parts)]]
 |-
 |Protein
@@ Line 12: / Line 21: @@
 |-
 |Cerulean
-|<span style="background:Cyan; color:black;"> Cyan </span>
+|style="background:Cyan" |Cyan
 |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_I757009'>BBa_I757009</A></HTML>
 |433
 |475
 |0.62
+|-
+|eCFP
+|style="background:Cyan" |Cyan
+|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1159302'>BBa_K1159302</A></HTML>
+|439
+|476
+|0.40
 |-
 |GFP
-|<span style="background:Lime; color:black;"> Green </span>
+|style="background:Lime" |Green
 |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K648013'>BBa_K648013</A></HTML>
 |484
@@ Line 26: / Line 42: @@
 |-
 |GFP
-|<span style="background:Lime; color:black;"> Green </span>
+|style="background:Lime" |Green
 |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K863120'>BBa_K863120</A></HTML>
 |484
@@ Line 33: / Line 49: @@
 |-
 |GFP
-|<span style="background:Lime; color:black;"> Green </span>
+|style="background:Lime" |Green
 |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K823039'>BBa_K823039</A></HTML>
 |484
@@ Line 40: / Line 56: @@
 |-
 |sfGFP
-|<span style="background:Lime; color:black;"> Green </span>
+|style="background:Lime" |Green
 |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1321337'>BBa_K1321337</A></HTML>
 |485
@@ Line 47: / Line 63: @@
 |-
 |Dronpa (photoactivatable)
-|<span style="background:Lime; color:black;"> Green </span>
+|style="background:GreenYellow" |GreenYellow
 |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1680006'>BBa_K1680006</A></HTML>
 |503
@@ Line 54: / Line 70: @@
 |-
 |mVenus
-|<span style="background:Yellow; color:black;"> Yellow </span>
+|style="background:Yellow" |Yellow
 |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_I757008'>BBa_I757008</A></HTML>
 |515
@@ Line 61: / Line 77: @@
 |-
 |sYFP2
-|<span style="background:Yellow; color:black;"> Yellow </span>
+|style="background:Yellow" |Yellow
 |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1159301'>BBa_K1159301</A></HTML>
 |514
 |527
 |0.61
-|-
-|eCFP
-|<span style="background:DeepPink; color:black;"> Cyan </span>
-|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1159302'>BBa_K1159302</A></HTML>
-|439
-|476
-|0.40
 |-
 |mRFP
-|<span style="background:red; color:black;"> Red </span>
+|style="background:Red" |Red
 |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1351021'>BBa_K1351021</A></HTML>
 |584
@@ Line 82: / Line 91: @@
 |-
 |mRFP
-|<span style="background:red; color:black;"> Red </span>
+|style="background:Red" |Red
 |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1615089'>BBa_K1615089</A></HTML>
 |584
@@ Line 89: / Line 98: @@
 |-
 |mKate2
-|<span style="background:red; color:black;"> Red </span>
+|style="background:Red" |Red
 |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K823029'>BBa_K823029</A></HTML>
 |588
 |633
-|
+|0.4
 |-
+|colspan="6"|Values for the fluorescent proteins were taken from [http://www.microscopyu.com/articles/livecellimaging/fpintro.html| microscopyu.com].
 |}
-Values for the fluorescent proteins were taken from http://www.microscopyu.com/articles/livecellimaging/fpintro.html.
 ==Luciferases==
 {| class="wikitable"
-!colspan="4"|Luciferases
+!colspan="6"|[[RFC25_"Freiburg_standard"_parts#Luciferases| Luciferases (click for all RFC25 parts)]]
 |-
 |Protein
@@ Line 113: / Line 121: @@
 |-
 |''Renilla'' luciferase
-|
+|not available yet
 |36
 |-
@@ Line 121: / Line 129: @@
 |-
 |''Gaussia'' luciferase
-|
+|not available yet
 |
 |-
@@ Line 127: / Line 135: @@
 ==Transmembrane domains==
+==Optogenetic parts==
 ==Linker domains==
 {| class="wikitable"
-!colspan="4"|Linker
+!colspan="6"|[[RFC25_"Freiburg_standard"_parts#Linker| Linker (click for all RFC25 parts)]]
 |-
 |Protein
@@ Line 156: / Line 166: @@
 ==Catalytic proteins==
 {| class="wikitable"
-!colspan="6"|Catalytic proteins
+!colspan="6"|[[RFC25_"Freiburg_standard"_parts#Catalytic_proteins| Catalytic proteins (click for all RFC25 parts)]]
 |-
 |Erythromycin Esterase Type II
@@ Line 197: / Line 207: @@
 ==Transcription regulators==
 {| class="wikitable"
-!colspan="6"|Transcription regulators
+!colspan="6"|[[RFC25_"Freiburg_standard"_parts#Transcription_regulators| Transcription regulators (click for all RFC25 parts)]]
 |-
 |VP16
@@ Line 205: / Line 215: @@
 ==Split proteins==
 {| class="wikitable"
-!colspan="6"|Split proteins
+!colspan="6"|[[RFC25_"Freiburg_standard"_parts#Split_proteins| Split proteins (click for all RFC25 parts)]]
 |-
 |TEV Protease
@@ Line 218: / Line 228: @@
 ==Binding proteins==
 {| class="wikitable"
-!colspan="6"|Binding proteins
+!colspan="6"|[[RFC25_"Freiburg_standard"_parts#Binding_proteins| Binding proteins (click for all RFC25 parts)]]
 |-
 |Fluorescein binding Anticalin
@@ Line 237: / Line 247: @@
 |Cellulose binding Domain
 |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K863110'>BBa_K863110</A></HTML>
+|}
+==Protease cleavage sites==
+{| class="wikitable"
+!colspan="6"|[[RFC25_"Freiburg_standard"_parts#Protease_cleavage_sites| Protease cleavage sites (click for all RFC25 parts)]]
+|-
+|Name
+|Sequence
+|BioBrick
+|-
+|TEV cleavage site
+|G-ENLYFQS-G
+|<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1159318'>BBa_K1159318</A></HTML>
+|-
 |}
 ==Affinity tags==
 {| class="wikitable"
-!colspan="6"|Affinity tags
+!colspan="6"|[[RFC25_"Freiburg_standard"_parts#Affinity_tags| Affinity tags (click for all RFC25 parts)]]
 |-
 |HA tag

Difference between revisions of "RFC25 "Freiburg standard" parts"

Latest revision as of 20:39, 16 May 2016

Contents

RFC25 BioBrick parts

RFC25 compatible BioBrick backbones

Fluorescent proteins

Luciferases

Transmembrane domains

Optogenetic parts

Linker domains

Catalytic proteins

Transcription regulators

Split proteins

Binding proteins

Protease cleavage sites

Affinity tags