Difference between revisions of "Part:BBa M36144:Experience"
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12/04/15
 
12/04/15
  
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This His tag was codon optimized for E.Coli and worked properly with both our constructs, one with  mature EP-B2 transformed E.Coli cells and one with Pro Ep-B2 transformed E.Coli cells. The His tag was successful in purifying the targeted protein (mature EP-B2 or Pro EP-B2). We had lots of fluorescence increase in the lysed cells assay for both the mature EP-B2 and Pro EP-B2, which shows a large amount of the protein was present, and having it purified amplified these results.
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This His tag was codon optimized for E.Coli and specific for the EP-B2 protein. It's a marker for the EP-B2 protein that can be used for identification. Another purpose of the His tag is protein purification, specifically The EP-B2 protein. When planning our experiments, we were going to do a Western Blot, however, we ended up not needing to do a Western Blot, because our other assays provided sufficient results. Since we did not do a Western Blot the His tag was not actually a necessary part of the construct. If we end up doing follow-up experiments this His tag will be necessary.

Latest revision as of 07:03, 5 December 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_M36144

User Reviews

UNIQcf0250553ca844f1-partinfo-00000000-QINU UNIQcf0250553ca844f1-partinfo-00000001-QINU

12/04/15

This His tag was codon optimized for E.Coli and specific for the EP-B2 protein. It's a marker for the EP-B2 protein that can be used for identification. Another purpose of the His tag is protein purification, specifically The EP-B2 protein. When planning our experiments, we were going to do a Western Blot, however, we ended up not needing to do a Western Blot, because our other assays provided sufficient results. Since we did not do a Western Blot the His tag was not actually a necessary part of the construct. If we end up doing follow-up experiments this His tag will be necessary.