Difference between revisions of "Part:BBa M36580:Experience"
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===Stanford Location=== | ===Stanford Location=== | ||
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| − | + | == Glycerol Stocks == | |
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Barcode #: 0133010047 | Barcode #: 0133010047 | ||
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Plasmid Name: qsc102 | Plasmid Name: qsc102 | ||
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Antibiotic resistance: kanamycin | Antibiotic resistance: kanamycin | ||
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DNA 2.0 gene #: 232006 | DNA 2.0 gene #: 232006 | ||
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Organism expressed in: E. coli | Organism expressed in: E. coli | ||
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Sensor | Sensor | ||
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Barcode #: 0133010047 | Barcode #: 0133010047 | ||
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Plasmid Names (double transformed): LasR + qsc102 | Plasmid Names (double transformed): LasR + qsc102 | ||
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Antibiotic resistance: ampicillin + kanamycin | Antibiotic resistance: ampicillin + kanamycin | ||
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DNA 2.0 gene #: 232005; 232006 | DNA 2.0 gene #: 232005; 232006 | ||
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Organism expressed in: E. coli | Organism expressed in: E. coli | ||
| − | Double Plasmid Actuator/Sensor | + | |
| + | Double Plasmid Actuator/Sensor | ||
===Applications of BBa_M36580=== | ===Applications of BBa_M36580=== | ||
| + | Used in conjunction with BBa_M36575 in BIOE44 at Stanford in attempts to create a ''P. aeruginosa'' biosensor. | ||
===User Reviews=== | ===User Reviews=== | ||
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| + | We have little data on the functionality of this construct since it was used in conjunction with BBa_M36575, which proved inviable. However, what we do know is that a low amount of fluorescence was observed in all cells with the qsc102 plasmid, despite a lack of LasR presence (Graph 1). The promoter is likely leaky, and transcribed the GFP gene following it even without presence of the LasR-PAI1 complex. | ||
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| + | [[File:Graph 1.png]] | ||
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| + | Graph 1. Normalized fluorescence levels for GFP expression in the double-transformed ''E. coli'' | ||
Latest revision as of 21:10, 7 December 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Stanford Location
Glycerol Stocks
Barcode #: 0133010047
Plasmid Name: qsc102
Antibiotic resistance: kanamycin
DNA 2.0 gene #: 232006
Organism expressed in: E. coli
Sensor
Barcode #: 0133010047
Plasmid Names (double transformed): LasR + qsc102
Antibiotic resistance: ampicillin + kanamycin
DNA 2.0 gene #: 232005; 232006
Organism expressed in: E. coli
Double Plasmid Actuator/Sensor
Applications of BBa_M36580
Used in conjunction with BBa_M36575 in BIOE44 at Stanford in attempts to create a P. aeruginosa biosensor.
User Reviews
UNIQ962fcfa496202f34-partinfo-00000000-QINU UNIQ962fcfa496202f34-partinfo-00000001-QINU
We have little data on the functionality of this construct since it was used in conjunction with BBa_M36575, which proved inviable. However, what we do know is that a low amount of fluorescence was observed in all cells with the qsc102 plasmid, despite a lack of LasR presence (Graph 1). The promoter is likely leaky, and transcribed the GFP gene following it even without presence of the LasR-PAI1 complex.
Graph 1. Normalized fluorescence levels for GFP expression in the double-transformed E. coli