Difference between revisions of "Part:BBa M36534"
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| − | This part was made in order to | + | This part was made in order to reduce arsenate to arsenite and enhance arsenite resistance in ''S. cerevisae.'' |
| − | DNA 2.0 | + | Construct uses DNA 2.0 plasmid backbone (pD1207) with GAL1 inducible (galactose-inducible/glucose-repressible) promoter, high copy number ORI, and kanamycin and histidine as selectable markers in E. coli and yeast, respectively. A self-cleaving 2A peptide sequence was inserted between ACR2 and ACR1 genes to allow for both genes to be transcribed. |
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Latest revision as of 07:02, 8 December 2015
Arsenate Bioremediation Actuator Plasmid Containing ACR 1 and ACR 2 Genes
This part was made in order to reduce arsenate to arsenite and enhance arsenite resistance in S. cerevisae.
Construct uses DNA 2.0 plasmid backbone (pD1207) with GAL1 inducible (galactose-inducible/glucose-repressible) promoter, high copy number ORI, and kanamycin and histidine as selectable markers in E. coli and yeast, respectively. A self-cleaving 2A peptide sequence was inserted between ACR2 and ACR1 genes to allow for both genes to be transcribed.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 873
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 873
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 873
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 873
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 873
- 1000COMPATIBLE WITH RFC[1000]