Difference between revisions of "Part:BBa M36533"
 
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<partinfo>BBa_M36533 short</partinfo>
 
<partinfo>BBa_M36533 short</partinfo>
  
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This part was made in order to enhance arsenic absorption and resistance in S. cerevisae.
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This part was made in order to increase arsenate uptake by ''S. cerevisiae.''
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DNA 2.0 Plasmid (pD1201) with GAL1 inducible promoter, high copy number, and Leucine as the selectable marker
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Construct uses DNA 2.0 plasmid backbone (pD1201) with GAL1 inducible (galactose-inducible/glucose-repressible) promoter, high copy number ORI, and ampicillin and leucine as selectable markers in E. coli and yeast, respectively.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 06:49, 8 December 2015

Arsenate Bioremediation Actuator Plasmid Containing PHO84 Gene

This part was made in order to increase arsenate uptake by S. cerevisiae.

Construct uses DNA 2.0 plasmid backbone (pD1201) with GAL1 inducible (galactose-inducible/glucose-repressible) promoter, high copy number ORI, and ampicillin and leucine as selectable markers in E. coli and yeast, respectively.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1647
    Illegal PstI site found at 1002
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1002
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1438
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1647
    Illegal PstI site found at 1002
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1647
    Illegal PstI site found at 1002
  • 1000
    COMPATIBLE WITH RFC[1000]