Difference between revisions of "Part:BBa M36580:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
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===Stanford Location===
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== Glycerol Stocks ==
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Barcode #: 0133010047
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Plasmid Name: qsc102
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Antibiotic resistance: kanamycin
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DNA 2.0 gene #: 232006
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Organism expressed in: E. coli
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Sensor
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Barcode #: 0133010047
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Plasmid Names (double transformed): LasR + qsc102
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Antibiotic resistance: ampicillin + kanamycin
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DNA 2.0 gene #: 232005; 232006
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Organism expressed in: E. coli
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Double Plasmid Actuator/Sensor
  
 
===Applications of BBa_M36580===
 
===Applications of BBa_M36580===
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Used in conjunction with BBa_M36575 in BIOE44 at Stanford in attempts to create a ''P. aeruginosa'' biosensor.
  
 
===User Reviews===
 
===User Reviews===
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We have little data on the functionality of this construct since it was used in conjunction with BBa_M36575, which proved inviable. However, what we do know is that a low amount of fluorescence was observed in all cells with the qsc102 plasmid, despite a lack of LasR presence (Graph 1). The promoter is likely leaky, and transcribed the GFP gene following it even without presence of the LasR-PAI1 complex.
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[[File:Graph 1.png]]
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Graph 1. Normalized fluorescence levels for GFP expression in the double-transformed ''E. coli''

Latest revision as of 21:10, 7 December 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.


Stanford Location

Glycerol Stocks

Barcode #: 0133010047

Plasmid Name: qsc102

Antibiotic resistance: kanamycin

DNA 2.0 gene #: 232006

Organism expressed in: E. coli

Sensor


Barcode #: 0133010047

Plasmid Names (double transformed): LasR + qsc102

Antibiotic resistance: ampicillin + kanamycin

DNA 2.0 gene #: 232005; 232006

Organism expressed in: E. coli

Double Plasmid Actuator/Sensor

Applications of BBa_M36580

Used in conjunction with BBa_M36575 in BIOE44 at Stanford in attempts to create a P. aeruginosa biosensor.

User Reviews

UNIQa3bd59488bf6bdef-partinfo-00000000-QINU UNIQa3bd59488bf6bdef-partinfo-00000001-QINU

We have little data on the functionality of this construct since it was used in conjunction with BBa_M36575, which proved inviable. However, what we do know is that a low amount of fluorescence was observed in all cells with the qsc102 plasmid, despite a lack of LasR presence (Graph 1). The promoter is likely leaky, and transcribed the GFP gene following it even without presence of the LasR-PAI1 complex.

Graph 1.png

Graph 1. Normalized fluorescence levels for GFP expression in the double-transformed E. coli