Difference between revisions of "Part:BBa M36242:Design"

 
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===Design Notes===
 
===Design Notes===
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Combination for allows for identification of EP-B2.
 
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Testing whether mature EP-B2 protein is folded properly and is then successfully secreted versus YebF.
  
 
===Source===
 
===Source===
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Mature EP-B2 DNA sequence and optimized E.Coli his-tag from  "Computational Design of an α-Gliadin Peptidase" . YebF secretion system, RBS, terminator and rhamnose promoter from Gene Designer 2.0
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Composite
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===References===
 
===References===

Latest revision as of 23:15, 22 October 2015


Mature EP-B2 YepF Secretion Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 262
    Illegal NheI site found at 285
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 414
    Illegal BglII site found at 1185
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 853
    Illegal AgeI site found at 937
    Illegal AgeI site found at 1276
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1604


Design Notes

Testing whether mature EP-B2 protein is folded properly and is then successfully secreted versus YebF.

Source

Mature EP-B2 DNA sequence and optimized E.Coli his-tag from "Computational Design of an α-Gliadin Peptidase" . YebF secretion system, RBS, terminator and rhamnose promoter from Gene Designer 2.0

References