Difference between revisions of "Part:BBa M36144:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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| + | 12/04/15 | ||
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| + | This His tag was codon optimized for E.Coli and specific for the EP-B2 protein. It's a marker for the EP-B2 protein that can be used for identification. Another purpose of the His tag is protein purification, specifically The EP-B2 protein. When planning our experiments, we were going to do a Western Blot, however, we ended up not needing to do a Western Blot, because our other assays provided sufficient results. Since we did not do a Western Blot the His tag was not actually a necessary part of the construct. If we end up doing follow-up experiments this His tag will be necessary. | ||
Latest revision as of 07:03, 5 December 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_M36144
User Reviews
UNIQ5aad3c954708959a-partinfo-00000000-QINU UNIQ5aad3c954708959a-partinfo-00000001-QINU
12/04/15
This His tag was codon optimized for E.Coli and specific for the EP-B2 protein. It's a marker for the EP-B2 protein that can be used for identification. Another purpose of the His tag is protein purification, specifically The EP-B2 protein. When planning our experiments, we were going to do a Western Blot, however, we ended up not needing to do a Western Blot, because our other assays provided sufficient results. Since we did not do a Western Blot the His tag was not actually a necessary part of the construct. If we end up doing follow-up experiments this His tag will be necessary.